Topics to be covered include:
-
Basics and
advance techniques in Laser Scanning Confocal Microscopy (LSCM), image
formation, 3-D reconstruction, and fluorphores labelling conditions for
various biological applications.
-
Selection of
filters for confocal fluorescence imaging, various immuno-cytochemical
techniques, photo bleaching and data analysis.
-
Localization of
proteins in live and fixed cells
-
Introduction of
SPM, basic principles of STM and AFM, AFM equipment description,
operating modes, AFM probes, etc.
-
SPM
applications in biological and life science
-
Practical
session: sample preparation, AFM set-up, imaging in contact and tapping
mode, high resolution imaging of selected samples (nanoparticles, carbon
nanotubes, biomolecules and soft surfaces)
-
SPM beyond
imaging: characterization of chemical composition, interfacial surface
properties, nanomechanical properties (nanoindentation), nanolithography
etc.
-
A new
developments in SPM: NSOM, combined confocal and Raman AFM
-
Practical
session: probing of surface properties by force measurements (examples
self-assembled monolayers, protein layers, and polymers).
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